[研究業績]

新たな情報スイッチ−beta Pix

新しい因子Noxa1によるNox活性化

架橋によるNADPH oxidase安定化

遺伝子融合による酵素安定化 　図

酵素複合体の構造

Racによる活性化と安定化　

O2- 発生デバイスの開発 図

アクチンの関わり 図

p40の役割　

貴重なタンパク質をつくる−アクチン

Nox2を調節する細胞内分子-スペルミン

ホスファチジン酸 図

Nox2への細胞内情報伝達

以前の研究　

b5還元酵素の構造と性質

[Keywords]

活性酸素／貪食細胞／分子認識／細胞内情報伝達／生体防御／遺伝子工学／融合タンパク質

[Publications]

Tamura M
Production of human beta-actin using a bacterial expression system with a cold schock vector
Current Protocols, in press (2017)

Tamura M., Kunihiro S., Hamashima Y., Yoshioka Y., Tone S., and Kameda K.
An improved superoxide-generating nanodevice for oxidative stress studies in cultured cells
Biotechnology Reports, 6, 45-50 (2015)

Yoshioka Y., Fujibayashi H., Kameda K., Kan D., Tone S., and Tamura M.
Induction of apoptosis in Caco-2 cells by exogenously added O2- produced by a nanodevic
Experimental Cell Research, 331, 408-415 (2015)

Kaito Y. kataoka R., Takechi K. Mihara T., and Tamura M.
Nox1 activation by bPix and the role of Ser-340 phosphorylation
FEBS Lett. 588, 1997-2002 (2014)
Kawano M., Ishii R., Yoshioka Y., Fukuda T. and Tamura M.
C-terminal truncation of Noxa1 greatly enhances its ability to activate Nox2 in a pure reconstitution system
Arch. Biochem. Biophys., 538 (2) 164-170 (2013)

Kawano M., Miyamoto K., Kaito Y., Sumimoto H., and Tamura M.
Noxa1 as a moderate activator of Nox2-based NADPH oxidase
Arch. Biochem. Biophys., 519 (1) 1-7 (2012)

Tamura M., Ito K., Kunihiro S., Yamasaki C., and Haragauchi M.
Production of human beta-actin and a mutant using a bacterial expression system
with a cold shock vector
Protein Expr. Purif., 78 (1) 1-5 (2011)

田 村 　実
TNF受容体シグナル伝達の新しいかたち
-リボフラビンキナーゼを介するNADPH オキシダーゼの調節-
蛋白質 核酸 酵素、vol.54, No.14, 1878 (2009)

田 村 実
外に出たO2-はどのように細胞内シグナルとなるのか？
蛋白質 核酸 酵素、vol.53, No.13, 1797 (2008)

Nisimoto, Y. Tsubouchi, R. Diebold, B.A., Qiao, A, Ogawa, H., Ohara, T., and Tamura, M.
Activation of NADPH oxidase 1 in tumor colon epithelial cell
Biochem.J., 415 (1), 57-65 (2008)

Tamura, M., Shiozaki, I., Ono, S., Miyano, K., Kunihiro, S., and Sasaki, T.
p40phox as an alternative organizer to p47phox in Nox 2 activation:
a new mechanism involving an interaction with p22phox
FEBS Lett., vol.581, 4533-4538 (2007)

田村　実
酸性リン脂質はNADPH オキシダーゼ活性化の影の主役
生化学, vol.78、No.11, 1083-1088（2006）

Tamura, M., Itoh, K., Akita H., Takano,K. Oku, S.
Identification of an actin-binding site in p47phox an organizer protein of NADPH oxidase
FEBS Lett., vol.580, 261-267 (2006)

Tamura, M., Nagasawa,T., Tange,T., Miyano,K., Kobayashi,S., Nakai,T., and Miura,S.
A new type of O2- generating tool for oxidative stress studies by remodeling neutrophil NADPHoxidase
J. Biotechnol. vol.120, 421-429 (2005)

宮野 佳、田村 実、住本 英樹
好中球による活性酸素生成の分子メカニズム
Inflamation and Regeneration、vol.25, 113-117 (2005)

Nisimoto,Y., Ogawa,H., Miyano,K., and Tamura, M.
Activation of flavoprotein domain of gp91phox upon interaction with a fusion protein between p67phox(1-210) and Rac
Biochemistry, vol.43, 9565ー9575 (2004)

Miyano,K., Kitahara, H., Ohmi,S., Kakinuma, K., Tamura, M.
Inactivation of neutrophil NADPH oxidase upon dilution and its prevention by cross-link and fusion of phox proteins
Arch. Biochem. Biophys., vol.431, 129-137 (2004)

Nagasawa,T., Ebisu,K., Inoue, Y. , Miyano,K., and Tamura, M.
A new role of Pro-73 of p47phox in the activation of neutrophil NADPH oxidase
Arch.Biochem.Biophys., vol.416, 92-100　(2003)

Miyano, K., Fukuda, H., Ebisu, K., and Tamura, M.
Remarkable stabilization of neutrophil NADPH oxidase using RacQ61L and a p67phox-p47phox fusion protein
Biochemistry, vol.42, 184-190 (2003)

Miyano, K., Ogasawara, S., Han, C-H. , Fukuda, H., and Tamura, M.
A fusion protein between rac and p67phox (1-210) reconstitutes NADPH oxidase with higher activity and stability than the individual components
Biochemistry, vol.40, 14089-14097 (2001)

Ebisu K, Nagasawa T, Watanabe K, Kakinuma K, Miyano K, and Tamura M.
Fused p47phox and p67phox truncations efficiently reconstitute NADPH oxidase with Higher activity and stability than the individual components
J. Biol. Chem., vol.276, 24498-24505 (2001)

Tamura, M., Kanno, M., and Kai, T.
Destabilization of neutrophil NADPH oxidase by ATP and other trinucleotides, and in prevention by Mg2+
Biochim.Biophys.Acta, vol.1510, 270-277 (2001)

Tamura, M., Kai, T., Tsunawaki, S., Lambeth, J.D., and Kameda, K.
Direct Iinteraction of actin with p47phox of neutrophil NADPH oxidase
Biochem.Biophys.Res.Commun., vol.276, 1186-1190 (2000)

Tamura, M., Kanno, M., and Endo, Y.
Deactivation of neutrophil NADPH oxidase by actin-depolymerizing agents in a cell-free system
Biochem.J., vol.349, 369-375 (2000)

Tamura, M. Yoshida, K., and Kataoka, K.
GTPgS-induced O2- generation in permeabilized neutrophils requires PKC and phospholipase C but not tyrosine kinase or phospholipase D
Arch.Biochem.Biophys., vol.361, 257-263 (1999)

Morimatsu, T., Kawagoshi, A., Yoshida, K., and Tamura, M.
Actin enhances the activation of neutrophil NADPH oxidase in a cell-free system and semi-recombinant system
Biochem.Biophys.Res.Commun., vol.230, 206-210 (1997)

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